Identification of FAM46D as a novel cancer/testis antigen using EST data and serological analysis

Bettoni, Fabiana
Filho, Fernando Camargo
Grosso, Daniela M.
Galante, Pedro A.F.
Parmigiani, Raphael B.
Geraldo, Murilo V.
Henrique-Silva, Flávio
Oba-Shinjo, Sueli M.
Marie, Suely K.N.
Soares, Fernando A.
Brentani, Helena P.
Simpson, Andrew J.G.
de Souza, Sandro J.
Camargo, Anamaria A.

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Publication date

September 2009

DOI

10.1016/j.ygeno.2009.06.001

Publisher

Elsevier Inc.

Abstract

AbstractCancer/testis Antigens (CTAs) are immunogenic proteins with a restricted expression pattern in normal tissues and aberrant expression in different types of tumors being considered promising candidates for immunotherapy. We used the alignment between EST sequences and the human genome sequence to identify novel CT genes. By examining the EST tissue composition of known CT clusters we defined parameters for the selection of 1184 EST clusters corresponding to putative CT genes. The expression pattern of 70 CT gene candidates was evaluated by RT-PCR in 21 normal tissues, 17 tumor cell lines and 160 primary tumors. We were able to identify 4 CT genes expressed in different types of tumors. The presence of antibodies against the protein e...