Analysis of the 5′ non-coding region of rat liver S-adenosylmethionine synthetase mRNA and comparison of the Mr deduced from the cDNA sequence and the purified enzyme
- Alvarez, Luis
- Asunción, Miryam
- Corrales, Fernando
- Pajares, Maria A.
- Mato, José M.
Publication date
September 1991
DOI Publisher
Published by Elsevier B.V. ISSN
0014-5793 Citation count (estimate)
42Abstract
AbstractA 3 kb cDNA coding for rat liver S-adenosylmethionine (AdoMet) synthetase has been isolated. The Mr of the protein has been unequivocally determined by cDNA sequencing and enzyme purification on a thiopropyl-Sepharose column. The length of the mRNA 5′ non-coding region has been defined by primer-extension analysis. The rat liver cloned cDNA has been also used to detect S-adenosylmethionine synthetase mRNA in human liver
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