The effect on actin ATPase of phalloidin and tetramethylrhodamine phalloidin
- Pinaev, George
- Schutt, Clarence E.
- Lindberg, Uno
Publication date
August 1995
DOI Publisher
Published by Elsevier B.V. ISSN
0014-5793Abstract
AbstractActin polymerization has been studied in the absence of excess nucleotide. Using G-actin ATP monomers, it was shown that mechanical shearing stimulates ATP hydrolysis. The procedures used enabled the detection of differential effects of phalloidin and tetramethylrhodamine-phalloidin, on the Pi-release step of the actin ATPase. It is concluded that tetramethylrhodamine, in contrast to phalloidin, accelerates Pi-release from actin filaments
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