SummaryHigh-throughput screening (HTS) of chemical libraries has become a critical tool in basic biology and drug discovery. However, its implementation and the adaptation of high-content assays to human embryonic stem cells (hESCs) have been hampered by multiple technical challenges. Here we present a strategy to adapt hESCs to HTS conditions, resulting in an assay suitable for the discovery of small molecules that drive hESC self-renewal or differentiation. Use of this new assay has led to the identification of several marketed drugs and natural compounds promoting short-term hESC maintenance and compounds directing early lineage choice during differentiation. Global gene expression analysis upon drug treatment defines known and novel pat...
<div><p>Differentiated cells from human embryonic stem cells (hESCs) provide an unlimited source of ...
Differentiated cells from human embryonic stem cells (hESCs) provide an unlimited source of cells fo...
AbstractMeticulous characterization of human embryonic stem cells (hESC) is critical to their eventu...
AbstractUnderstanding the complex mechanisms that govern the fate decisions of human embryonic stem ...
Understanding the complex mechanisms that govern the fate decisions of human embryonic stem cells (h...
The increasing use of human pluripotent stem cells (hPSCs) as a source of cells for drug discovery, ...
Cell-permeable compounds provide a convenient and efficient approach to manipulate biological proces...
AbstractSerum-free differentiation protocols of human embryonic stem cells (hESCs) offer the ability...
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerativ...
SummaryRecent evidence indicates that mouse and human embryonic stem cells (ESCs) are fixed at diffe...
High throughput screens (HTS) of biological and synthetic molecules has become a useful tool in drug...
Human embryonic stem cells (hESCs) offer the potential to develop in vitro protocols for the generat...
Human pluripotent stem cells (hPSCs) are heralded to transform regenerative medicine and drug discov...
Naive human embryonic stem cells (hESCs) have been isolated that more closely resemble the pre-impla...
SummaryChemical biology methods such as high-throughput screening (HTS) and affinity-based target id...
<div><p>Differentiated cells from human embryonic stem cells (hESCs) provide an unlimited source of ...
Differentiated cells from human embryonic stem cells (hESCs) provide an unlimited source of cells fo...
AbstractMeticulous characterization of human embryonic stem cells (hESC) is critical to their eventu...
AbstractUnderstanding the complex mechanisms that govern the fate decisions of human embryonic stem ...
Understanding the complex mechanisms that govern the fate decisions of human embryonic stem cells (h...
The increasing use of human pluripotent stem cells (hPSCs) as a source of cells for drug discovery, ...
Cell-permeable compounds provide a convenient and efficient approach to manipulate biological proces...
AbstractSerum-free differentiation protocols of human embryonic stem cells (hESCs) offer the ability...
Human embryonic stem cell (hESC) research provides promising prospects for the future of regenerativ...
SummaryRecent evidence indicates that mouse and human embryonic stem cells (ESCs) are fixed at diffe...
High throughput screens (HTS) of biological and synthetic molecules has become a useful tool in drug...
Human embryonic stem cells (hESCs) offer the potential to develop in vitro protocols for the generat...
Human pluripotent stem cells (hPSCs) are heralded to transform regenerative medicine and drug discov...
Naive human embryonic stem cells (hESCs) have been isolated that more closely resemble the pre-impla...
SummaryChemical biology methods such as high-throughput screening (HTS) and affinity-based target id...
<div><p>Differentiated cells from human embryonic stem cells (hESCs) provide an unlimited source of ...
Differentiated cells from human embryonic stem cells (hESCs) provide an unlimited source of cells fo...
AbstractMeticulous characterization of human embryonic stem cells (hESC) is critical to their eventu...