Effect Comparison of different formulation of Dang–Gui–Bu–Xu–Tang on myelosuppression mouse

AbstractObjectiveTo compare effect of different formulation of Dang-Gui-Bu-Xu-Tang(DGBXT) on myelosuppression mouse.MethodsHPLC was used to measure active ingredients of two DGBXT formulations. Hemopoesic function of bone marrow was measured by hemopoietic progenitor cell culture and peipheral blood count. And hemopoietic factors in bone marrow were tested by ELISA.ResultsThe content level of astragaloside A in granule formulation was higher than that in decoction and the content ratio of active ingredient was close to 5 : 1. Two DGBXD formulations could significantly improve amount of peripheral blood cells, and bone marrow cells of bone marrow suppression mice (P<0.05). DGBXD granule formulation significantly increased the colony quantity of all progenitor cell lines and amount of G2/M and S phase cells (P<0.05). It also significantly decreased amount of G0/G1 phase cells in the bone marrow and was more effective (P