Synthesis of carbon nanosheet from barley and its use as non-enzymatic glucose biosensor

AbstractIn this work, carbon nanosheet (CNS) based electrode was designed for electrochemical biosensing of glucose. CNS has been obtained by the pyrolysis of barley at 600–750°C in a muffle furnace; it was then purified and functionalized. The CNS has been characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopic techniques. The electrochemical activity of CNS-based electrode was investigated by linear sweep voltammetry (LSV) and square wave voltammetry (SWV), for the oxidation of glucose in 0.001M H2SO4 (pH 6.0). The linear range of the sensor was found to be 10−4–10−6M (1–100µM) within the response time of 4s. Interestingly, its sensitivity reached as high as ~26.002±0.01μA/μMcm2. Electrochemical experiments revealed that the proposed electrode offered an excellent electrochemical activity towards the oxidation of glucose and could be applied for the construction of non-enzymatic glucose biosensors