Primary structure of the α-subunit of vacuolar-type Na+-ATPase inEnterococcus hirae Amplification of a 1000-bp fragment by polymerase chain reaction

AbstractA 1000-bp fragment ofEnterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na+-ATPase α-subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na+-ATPase α-subunit is highly homologous to the corresponding sequences of large (α) subunits of vacuolar (archaebacterial) type H+-ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97–101] that the Na+-ATPase of this organism belongs to the vacuolar-type ATPase