Occurrence of lipid and phorbol ester activated protein kinase in wheat cells

AbstractChromatography of the 30000 × g supernatant of wheat cell homogenates on DEAE-cellulose yielded a fraction whose protein kinase activity was specifically stimulated by phosphatidylserine plus phorbol ester or phosphatidylserine plus diolein. Such a characteristic activation pattern established this protein kinase as being a kinase C, an enzyme which plays an important role in the regulation of cell growth and differentiation in animal cells