Reconstitution of photosynthetic oxygen evolving activity by rebinding of 33 kDa protein to CaCl2-extracted PS II particles

AbstractOn extracting the O2-evolving Photosystem II (PS II) particles with CaCl2 solution, three proteins of 33, 24 and 16 kDa were completely liberated from the particles concomitant with total inactivation of O2 evolution, but most of the Mn atoms present in the initial particles were preserved unaffected being still associated with the particles. On reading the extracted proteins to the extracted particles, a considerable amount of 33 kDa protein rebound to the particles concomitant with significant reconstitution of O2-evolving activity