Simultaneous detection of gastrointestinal pathogens with a multiplex Luminex-based molecular assay in stool samples from diarrhoeic patients
- Mengelle, C.
- Mansuy, J.M.
- Prere, M.F.
- Grouteau, E.
- Claudet, I.
- Kamar, N.
- Huynh, A.
- Plat, G.
- Benard, M.
- Marty, N.
- Valentin, A.
- Berry, A.
- Izopet, J.
DOIAbstract
AbstractWe have evaluated the multiplex molecular method xTAG® Gastrointestinal Panel (GPP) for detecting pathogens in stool samples of diarrhoeic patients. We collected 440 samples from 329 patients (male:female ratio of 1.2:1), including 102 immunosuppressed adults, 50 immunosuppressed children, 56 children attending the neonatal unit and 121 children attending the emergency unit. Of these, 176 samples from 162 patients were xTAG® GPP positive (102 viruses, 61 bacteria and 13 parasites) and the assay was more sensitive than the conventional test for detecting rotavirus (p <0.01), noroviruses (p <0.0001), Salmonella spp. (p <0.001), Campylobacter spp. (p <0.001) and toxigenic Clostridium difficile (p 0.005). The predominant pathogens were ...
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