Glutamine synthetase from the plant fraction of Phaseolus root nodules Purification of the mRNA and in vitro synthesis of the enzyme

AbstractAntiserum raised to glutamine synthetase (GS) from the plant fraction of Phaseolus vulgaris root nodules was used to immunoprecipitate in vitro translation products of nodule and root poly(A)+ RNA. The results showed that the large increase in GS activity which occurs during nodulation is due to an increased supply of GS mRNA. A 3-fold enrichment of GS mRNA was obtained by centrifugation of total nodule poly. (A)+ RNA on dimethylsulphoxide-formamide-containing sucrose density gradients and purification to near homogeneity was achieved by immunopurification of nodule polysomes