AbstractNew lines of evidence suggest that volatile anesthetics interact specifically with proteins. Direct binding analysis, however, has been largely limited to soluble proteins. In this study, specific interaction was investigated between isoflurane, a clinically important volatile anesthetic, and membrane-bound nicotinic acetylcholine receptors (nAChRs) from Torpedo electroplax, using 19F nuclear magnetic resonance spectroscopy and gas chromatography. The receptors were reconstituted into 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles. After correcting for nonspecific partitioning into the lipid, the equilibrium dissociation constant, Kd, of isoflurane binding to nAChR at 15°C was found to be 0.36±0.03mM. This value is w...
AbstractComputational methods designed to predict and visualize ligand protein binding interactions ...
Despite the widespread clinical use of anesthetics since the 19th century, a clear understanding of ...
The interactions of the local anesthetic tetracaine with multilamellar dispersions of dimyristoylpho...
AbstractNew lines of evidence suggest that volatile anesthetics interact specifically with proteins....
AbstractThe molecular basis of anesthetic interaction with membrane proteins has been explored via d...
AbstractThe trend of evidence suggests that general anesthetics act directly on proteins in the neur...
AbstractThe α4β2 nicotinic acetylcholine receptor (nAChR) has significant roles in nervous system fu...
AbstractMolecular dynamics simulations have been performed to investigate the partitioning of the vo...
[Objectives] To investigate the interaction of a general anesthetic with biological membranes, we me...
AbstractThe nicotinic acetylcholine receptor (nAChR) is an important therapeutic target for a wide r...
The nicotinic acetylcholine receptor (nAChR) is an important therapeutic target for a wide range of ...
Acetylcholine, the agonist for the nicotinic acetylcholine receptor, has been observed directly when...
The relationship between the structure and function of a series of nicotinic cholinergic agonists ha...
The nicotinic acetylcholine receptor (nAChR) mediates fast signal transduction in peripheral and cen...
AbstractThe membrane location of the local anesthetics (LA) lidocaine, dibucaine, tetracaine, and pr...
AbstractComputational methods designed to predict and visualize ligand protein binding interactions ...
Despite the widespread clinical use of anesthetics since the 19th century, a clear understanding of ...
The interactions of the local anesthetic tetracaine with multilamellar dispersions of dimyristoylpho...
AbstractNew lines of evidence suggest that volatile anesthetics interact specifically with proteins....
AbstractThe molecular basis of anesthetic interaction with membrane proteins has been explored via d...
AbstractThe trend of evidence suggests that general anesthetics act directly on proteins in the neur...
AbstractThe α4β2 nicotinic acetylcholine receptor (nAChR) has significant roles in nervous system fu...
AbstractMolecular dynamics simulations have been performed to investigate the partitioning of the vo...
[Objectives] To investigate the interaction of a general anesthetic with biological membranes, we me...
AbstractThe nicotinic acetylcholine receptor (nAChR) is an important therapeutic target for a wide r...
The nicotinic acetylcholine receptor (nAChR) is an important therapeutic target for a wide range of ...
Acetylcholine, the agonist for the nicotinic acetylcholine receptor, has been observed directly when...
The relationship between the structure and function of a series of nicotinic cholinergic agonists ha...
The nicotinic acetylcholine receptor (nAChR) mediates fast signal transduction in peripheral and cen...
AbstractThe membrane location of the local anesthetics (LA) lidocaine, dibucaine, tetracaine, and pr...
AbstractComputational methods designed to predict and visualize ligand protein binding interactions ...
Despite the widespread clinical use of anesthetics since the 19th century, a clear understanding of ...
The interactions of the local anesthetic tetracaine with multilamellar dispersions of dimyristoylpho...