Direct measurement of oscillatory generation of superoxide anions by single phagocytes

AbstractPhagocytic cells such as neutrophils generate superoxide anions (O2−) within phagocytic vacuoles for killing and digesting microorganisms. Here we report the simultaneous observation of morphological changes and O2− generation in single phagocytic cells during phagocytosis. Point stimulation of a cell by contact with an opsonized microelectrode at the cell surface induced significant deformation to engulf the electrode, and also induced the O2− generation which was measured by the electrode. Periodic fluctuations in the magnitude of the O2− generation were observed in the time course. These oscillations may be caused by metabolic regulation of the formation of NADPH, which is the substrate for the O2− generation