Somatostatin and α2-adrenergic agonists selectively inhibit vasopressin-induced cyclic AMP accumulation in MDCK cells

AbstractThe effect of somatostatin and α2-adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in denned medium. These hormones inhibited vasopressin-induced cyclic AMP formation, without affecting either the basal or the glucagon- and prostaglandin E2-stimulated level. Pretreating the cells with pertussis toxin, or incubating them with MnCl2 at a low concentration reversed the effect of somatostatin and α2-agonists. These results suggest that somatostatin and norepinephrine could selectively modulate the renal effect of vasopressin, via the inhibitory regulatory subunit (Ni) of adenylate cyclase