AbstractMicro-algae have received considerable interest as a potential feedstock for producing sustainable transport fuels (biofuels). The perceived benefits provide the underpinning rationale for much of the public support directed towards micro-algae research. Here we examine three aspects of micro-algae production that will ultimately determine the future economic viability and environmental sustainability: the energy and carbon balance, environmental impacts and production cost. This analysis combines systematic review and meta-analysis with insights gained from expert workshops.We find that achieving a positive energy balance will require technological advances and highly optimised production systems. Aspects that will need to be addre...