AbstractIn this work, we present a new model of stress-induced premature senescence obtained by exposing human fibroblasts (WI-38) at early passages (passages 2–4) to a single sub-cytotoxic dose of UVB (200mJ/cm2). We show that this treatment leads to the appearance of several biomarkers of senescence such as enlarged and flattened cell morphology, the presence of nuclear heterochromatic foci and β-galactosidase activity. Furthermore, we demonstrate that a mild ROS production and p53 activation are upstream events required for the induction of premature senescence. Our method represents an alternative in vitro model in photoaging research and could be used to test potential anti-photoaging compounds
Senescent cells are heterogenous populations of cells that have undergone phenotypical changes chara...
Secretory pathway calcium ATPase 1 (SPCA1) is a calcium pump localized specifically to the Golgi. It...
AbstractAfter a limited number of population doublings (PDs), cultures of normal mammalian diploid c...
AbstractIn this work, we present a new model of stress-induced premature senescence obtained by expo...
Ultraviolet B (UVB) induces morphological and functional changes of the skin. This study investigate...
AbstractHuman diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of sen...
Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidati...
Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidati...
Modern understanding of aging is based on the accumulation of cellular damage during one’s life span...
Background: Photoaging is mainly caused by ultraviolet radiations inasmuch they can damage the DNA, ...
Background/Aims: Ultraviolet B (UVB) irradiation alters multiple molecular pathways in the skin, the...
Chronic exposure to UVR is known to disrupt tissue homeostasis, accelerate the onset of age-related ...
Objective To establish an efficient skin photoaging model in HaCaT cells and investigate the possibl...
AbstractCellular senescence is an extremely stable form of cell cycle arrest that limits the prolife...
Although UVB radiation is mainly absorbed by the epidermis, ~5–10% of its photons reach and affect t...
Senescent cells are heterogenous populations of cells that have undergone phenotypical changes chara...
Secretory pathway calcium ATPase 1 (SPCA1) is a calcium pump localized specifically to the Golgi. It...
AbstractAfter a limited number of population doublings (PDs), cultures of normal mammalian diploid c...
AbstractIn this work, we present a new model of stress-induced premature senescence obtained by expo...
Ultraviolet B (UVB) induces morphological and functional changes of the skin. This study investigate...
AbstractHuman diploid fibroblasts (HDFs) exposed to subcytotoxic stress display many features of sen...
Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidati...
Human skin exposure to ultraviolet B (UVB) radiation can result in acute photodamage through oxidati...
Modern understanding of aging is based on the accumulation of cellular damage during one’s life span...
Background: Photoaging is mainly caused by ultraviolet radiations inasmuch they can damage the DNA, ...
Background/Aims: Ultraviolet B (UVB) irradiation alters multiple molecular pathways in the skin, the...
Chronic exposure to UVR is known to disrupt tissue homeostasis, accelerate the onset of age-related ...
Objective To establish an efficient skin photoaging model in HaCaT cells and investigate the possibl...
AbstractCellular senescence is an extremely stable form of cell cycle arrest that limits the prolife...
Although UVB radiation is mainly absorbed by the epidermis, ~5–10% of its photons reach and affect t...
Senescent cells are heterogenous populations of cells that have undergone phenotypical changes chara...
Secretory pathway calcium ATPase 1 (SPCA1) is a calcium pump localized specifically to the Golgi. It...
AbstractAfter a limited number of population doublings (PDs), cultures of normal mammalian diploid c...