Effect of grapeseed oil on diazinon-induced physiological and histopathological alterations in rats

AbstractThe pollution of environment by toxic chemicals is a global and chronic problem. Human health risk due to exposure to chemical pollutants is constantly increasing. Pesticides form major toxic chemicals in environment. Scientifically, there is an obviously correlation between the exposure to pesticides and appearance of many diseases. Currently, the significance of natural products for health and medicine has been formidable. The present study investigated the effect of grapeseed oil in male rats exposed to diazinon. The experimental rats were divided into five groups. The rats of the first group were served as control. The experimental animals of the second group were exposed to diazinon (DZN). The animals of the third group were supplemented with grapeseed oil and treated with DZN. The rats of the fourth group were supplemented with grapeseed oil. The experimental rats of the fifth group were supplemented with corn oil. Hematobiochemical and histopathological evaluations were chosen as indicators of DZN toxicity and protective role of grapeseed oil. In rats exposed only to DZN, the levels of serum glucose, triglycerides, cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, creatinine, urea nitrogen, uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase and lactate dehydrogenase were statistically increased, while the level of serum total protein was significantly decreased. Moreover, the histopathological evaluations of the liver, kidney and testis showed that DZN causes several severe alterations. Pretreatment with grapeseed oil exhibited a protective role against DZN toxicity which confirmed by the inhibition of hematobiochemical and histopathological changes due to DZN exposure. Additionally, the present study suggests that the effect of grapeseed oil supplementation against DZN toxicity may be attributed to the antioxidant role of its constituents