The Photochemical Cycle of Halorhodopsin Absolute Spectra of Intermediates Obtained by Flash Photolysis and Fast Difference Spectra Measurements

Results of experiments using flash photolysis and fast difference spectroscopy suggest an extended version of the earlier published scheme of the photochemical cycle of halorhodopsin. Detailed experimental verification of the suggested photocycle is given. Due to the high resolution of the time-resolved difference spectra, absolute spectra of the intermediates in the photocycle were derived, allowing the interpretation of complex kinetic absorbance changes