Association Rates of Membrane-Coupled Cell Adhesion Molecules

AbstractThus far, understanding how the confined cellular environment affects the lifetime of bonds, as well as the extraction of complexation rates, has been a major challenge in studies of cell adhesion. Based on a theoretical description of the growth curves of adhesion domains, we present a new (to our knowledge) method to measure the association rate kon of ligand-receptor pairs incorporated into lipid membranes. As a proof of principle, we apply this method to several systems. We find that the kon for the interaction of biotin with neutravidin is larger than that for integrin binding to RGD or sialyl Lewisx to E-selectin. Furthermore, we find kon to be enhanced by membrane fluctuations that increase the probability for encounters between the binders. The opposite effect on kon could be attributed to the presence of repulsive polymers that mimic the glycocalyx, which points to two potential mechanisms for controlling the speed of protein complexation during the cell recognition process