AbstractAddition of poly(A) to the 3′ ends of cleaved pre-mRNA is essential for mRNA maturation and is catalyzed by Pap1 in yeast. We have previously shown that a non-viable Pap1 mutant lacking the first 18 amino acids is fully active for polyadenylation of oligoA, but defective for pre-mRNA polyadenylation, suggesting that interactions at the N-terminus are important for enzyme function in the processing complex. We have now identified proteins that interact specifically with this region. Cft1 and Pta1 are subunits of the cleavage/polyadenylation factor, in which Pap1 resides, and Nab6 and Sub1 are nucleic-acid binding proteins with known links to 3′ end processing. Our results suggest a novel mechanism for controlling Pap1 activity, and p...
Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by ...
Most eukaryotic mRNAs are subject to considerable post-transcriptional modification, including cappi...
3' polyadenylation is a key step in eukaryotic mRNA biogenesis. In mammalian cells, this process is ...
AbstractAddition of poly(A) to the 3′ ends of cleaved pre-mRNA is essential for mRNA maturation and ...
In eukaryotic cells, newly synthesized mRNAs acquire a poly(A) tail that plays several fundamental r...
The poly(A) tail of an mRNA is believed to influence the initiation of translation, and the rate at ...
Posttranscriptional maturation of the 3\u27 end of eukaryotic pre-mRNAs occurs as a three-step pathw...
PAN, a yeast poly(A) nuclease, plays an important nuclear role in the posttranscriptional maturation...
AbstractRecent studies have uncovered new connections between the enzymes of mRNA 3′ end processing ...
Almost all eukaryotic messenger RNAs (mRNAs) have a polyadenosine (polyA) tail at their 3′ end that ...
Cleavage and polyadenylation factor (CPF) is a multi‐protein complex that functions in pre‐mRNA 3′‐e...
3' end formation of most eukaryotic mRNAs is dependent on the assembly of a ~1.5 MDa multiprotein co...
Cleavage and polyadenylation factor (CPF) is a multi‐protein complex that functions in pre‐mRNA 3′‐e...
International audiencePolyadenylation is the second step in 3' end formation of most eukaryotic mRNA...
AbstractWe have identified an essential gene, called FIP1, encoding a 327 amino acid protein interac...
Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by ...
Most eukaryotic mRNAs are subject to considerable post-transcriptional modification, including cappi...
3' polyadenylation is a key step in eukaryotic mRNA biogenesis. In mammalian cells, this process is ...
AbstractAddition of poly(A) to the 3′ ends of cleaved pre-mRNA is essential for mRNA maturation and ...
In eukaryotic cells, newly synthesized mRNAs acquire a poly(A) tail that plays several fundamental r...
The poly(A) tail of an mRNA is believed to influence the initiation of translation, and the rate at ...
Posttranscriptional maturation of the 3\u27 end of eukaryotic pre-mRNAs occurs as a three-step pathw...
PAN, a yeast poly(A) nuclease, plays an important nuclear role in the posttranscriptional maturation...
AbstractRecent studies have uncovered new connections between the enzymes of mRNA 3′ end processing ...
Almost all eukaryotic messenger RNAs (mRNAs) have a polyadenosine (polyA) tail at their 3′ end that ...
Cleavage and polyadenylation factor (CPF) is a multi‐protein complex that functions in pre‐mRNA 3′‐e...
3' end formation of most eukaryotic mRNAs is dependent on the assembly of a ~1.5 MDa multiprotein co...
Cleavage and polyadenylation factor (CPF) is a multi‐protein complex that functions in pre‐mRNA 3′‐e...
International audiencePolyadenylation is the second step in 3' end formation of most eukaryotic mRNA...
AbstractWe have identified an essential gene, called FIP1, encoding a 327 amino acid protein interac...
Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by ...
Most eukaryotic mRNAs are subject to considerable post-transcriptional modification, including cappi...
3' polyadenylation is a key step in eukaryotic mRNA biogenesis. In mammalian cells, this process is ...