The pyrrolopyrimidine U101033E is a potent free radical scavenger and prevents Fe(II)-induced lipid peroxidation in synaptosomal membranes
- Lauderback, Christopher M.
- Breier, Adam M.
- Hackett, Janna
- Varadarajan, Sridhar
- Goodlett-Mercer, Jessica
- Butterfield, D.Allan
DOIAbstract
AbstractThe pyrrolopyrimidine U101033E is a therapeutic compound potentially useful in stroke, head injury and other oxidative stress conditions. Electron paramagnetic resonance (EPR) techniques of spin labeling and spin trapping in conjunction with measures of lipid and protein oxidation have been used to investigate the proposed antioxidant capacity of U101033E. We report potent antioxidant activity of this agent in aqueous cell-free solution as measured by spin trapping. U101033E significantly (P<0.005) reduces the formation of the EPR active spin trap N-t-butyl-α-phenylnitrone (PBN)-radical adduct by 17.1% at a concentration of 1 μM, four orders of magnitude less than the concentration of PBN. As measured by the decrease in signal inten...
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