Phosphatidylcholine-phospholipase C mediates the induction of nerve growth factor in cultured glial cells
- Laviada, Inés D.
- Baudet, Cristel
- Galve-Roperh, Ismael
- Naveilhan, Philippe
- Brachet, Philippe
Publication date
May 1995
DOI Publisher
Published by Elsevier B.V. ISSN
0014-5793 Citation count (estimate)
19Abstract
AbstractAddition of phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) to cultured glial cells increased the levels of nerve growth factor (NGF) mRNA and the amount of cell-secreted NGF. The effect of PC-PLC was 2.5 times higher than that elicited by 4β-phorbol 12β-myristate 13α-acetate. In cells in which protein kinase C (PKC) was fully inhibited or downregulated, the effect of PC-PLC was reduced - though still evident - and similar to that exerted by sphingosine. Results thus indicate that PC-PLC induces the synthesis of NGF by glial cells by a PKC-dependent and PKC-independent mechanisms
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