Occurrence of a Tombusvirus on Erigeron canadensis L.

Iz listova zaraženih primjeraka korovne biljke Erigeron canadensis L. koji su rasli na nekoliko lokaliteta u Sarajevu izoliran je virus iz skupine tombusvirusi. Identifikacija virusa izvršena je na osnovi reakcije pokusnih biljaka, analize ultratankih presjeka kroz zaraženo tkivo, morfologije i veličine virusnih čestica, te na osnovi seroloških reakcija metodom dvostruke imunodifuzije u agarskom gelu i čvrsto fazne imunoelektronske mikroskopije (SPIEM). U serološkim pokusima upotrijebljen je imuni serum protiv špinatskog soja virusa grmolike kržljavosti rajčice. Ovim je radom vrsta E. canadensis prvi put zabilježena kao prirodni domaćin jednog tombusvirusa.Many samples of the species Erigeron canadensis L. with marked symptoms of viral disease were found in many locations in Sarajevo area, Yugoslavia. A virus belonging to the group of tombusviruses was isolated from infected plants. The identification of the virus is based on reactions of diagnostic herbaceous species, characteristic cytopathogenic changes, morphology and size of viral particles, serological test of double diffusion in agar gel, and immune electron microscopy by »trapping« method (SPIEM). In serological test the serum against spinach strain of tomato bushy stunt virus was used. This experiment, has shown for the first time that the species Enigeron canadensis is a natural host plant for a tombusvirus