Transformation of Saccharomyces cerevisiae spheroplasts by high electric pulse

AbstractAn electric pulse was applied to transformation of yeast cell. Saccharomyces cerevisiae D13-1A (a his3-532 trpl gal2) was used as a recipient strain for plasmid YRp7. Spheroplasts of S.cerevisiae D13-1A were placed under an electric pulse in the presence of YRp7. Transformants were observed when the pulse height was above 5 kVcm. The number of transformants increased with increasing pulse height and 945 transformants per ng DNA were obtained at 10 kVcm. Introduction of DNA might be caused by breakdown of the cell membrane under high electric voltage.TransformationSaccharomyces cerevisiaeSpheroplastPlasmidElectric puls