AbstractA plasmid pAc373GM-CSF was constructed and co-transfected into Spodoptera frugiperda (Sf9) cells with wild-type Autographa californica nuclear polyhedrosis virus (AcNPV) DNA. The recombinant virus vAc373GM-CSF was identified and purified by several rounds of plaque hybridization. By assaying the culture medium, we demonstrated recombinant virus infected Sf9 cells expressing hGM-CSF. Recombinant hGM-CSFs with apparent molecular masses of 14.5, 15.5 and 16.5 kDa were detected by the Western blot method. All 3 forms have biological activity of hGM-CSF. Following N-glycanase treatment, a single band of 14.5–15.5 kDa appeared in SDS-PAGE. Western blot analysis of expression in Sf9 cells treated with tunicamycin revealed only the presence...
Using a transfer vector derived from Bombyx mori nuclear polyhedrosis virus (BmNPV), we have constru...
AbstractWe have generated recombinant baculoviruses for expression of the green fluorescent protein ...
Baculoviruses are insect-specific pathogens, producing occlusion bodies containing enveloped virus p...
AbstractA plasmid pAc373GM-CSF was constructed and co-transfected into Spodoptera frugiperda (Sf9) c...
The baculovirus expression system employing Autagrapha californica nuclear polyhidrosis virus and Sp...
In recent years, the baculovirus expression vector system has become a well-established and popular ...
AbstractAutographa californica multicapsid nucleopolyhedrovirus (AcMNPV) is the best-studied baculov...
Generating large amounts of recombinant protein in transgenic animals is often challenging and has a...
The Spodoptera litura nucleopolyhedrovirus (SpltNPV) gp37 fusion gene was inserted into the transfer...
The gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Auto...
We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda ...
Granulocyte colony stimulating factor (G-CSF) is a hematopoietic cytokine that stimulates bone marro...
AbstractThe understanding of the structure and function of cGMP-dependent protein kinase (cGMP kinas...
Generating large amounts of recombinant protein in transgenic animals is often challenging and has...
The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid ...
Using a transfer vector derived from Bombyx mori nuclear polyhedrosis virus (BmNPV), we have constru...
AbstractWe have generated recombinant baculoviruses for expression of the green fluorescent protein ...
Baculoviruses are insect-specific pathogens, producing occlusion bodies containing enveloped virus p...
AbstractA plasmid pAc373GM-CSF was constructed and co-transfected into Spodoptera frugiperda (Sf9) c...
The baculovirus expression system employing Autagrapha californica nuclear polyhidrosis virus and Sp...
In recent years, the baculovirus expression vector system has become a well-established and popular ...
AbstractAutographa californica multicapsid nucleopolyhedrovirus (AcMNPV) is the best-studied baculov...
Generating large amounts of recombinant protein in transgenic animals is often challenging and has a...
The Spodoptera litura nucleopolyhedrovirus (SpltNPV) gp37 fusion gene was inserted into the transfer...
The gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Auto...
We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda ...
Granulocyte colony stimulating factor (G-CSF) is a hematopoietic cytokine that stimulates bone marro...
AbstractThe understanding of the structure and function of cGMP-dependent protein kinase (cGMP kinas...
Generating large amounts of recombinant protein in transgenic animals is often challenging and has...
The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid ...
Using a transfer vector derived from Bombyx mori nuclear polyhedrosis virus (BmNPV), we have constru...
AbstractWe have generated recombinant baculoviruses for expression of the green fluorescent protein ...
Baculoviruses are insect-specific pathogens, producing occlusion bodies containing enveloped virus p...