Two-Photon Fluorescence Lifetime Imaging of the Skin Stratum Corneum pH Gradient

AbstractTwo-photon fluorescence lifetime imaging is used to identify microdomains (1–25μm) of two distinct pH values within the uppermost layer of the epidermis (stratum corneum). The fluorophore used is 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF), whose lifetime τ (pH 4.5, τ=2.75ns; pH 8.5, τ=3.90ns) is pH dependent over the pH range of the stratum corneum (pH 4.5 to pH 7.2). Hairless mice (SKH1-hrBR) are used as a model for human skin. Images (≤50μm×50μm) are acquired every 1.7μm from the stratum corneum surface to the first viable layer (stratum granulosum). Acidic microdomains (average pH 6.0) of variable size (∼1μm in diameter with variable length) are detected within the extracellular matrix of the stratum corneum, whereas the intracellular space of the corneocytes in mid-stratum corneum (25μm diameter) approaches neutrality (average pH 7.0). The surface is acidic. The average pH of the stratum corneum increases with depth because of a decrease in the ratio of acidic to neutral regions within the stratum corneum. The data definitively show that the stratum corneum acid mantle results from the presence of aqueous acidic pockets within the lipid-rich extracellular matrix