Motif statistics

AbstractWe present a complete analysis of the statistics of number of occurrences of a regular expression pattern in a random text. This covers “motifs” widely used in computational biology. Our approach is based on: (i) classical constructive results in automata and formal language theory; (ii) analytic combinatorics that is used for deriving asymptotic properties from generating functions; (iii) computer algebra in order to determine generating functions explicitly, analyse generating functions and extract coefficients efficiently. We provide constructions for overlapping or non-overlapping matches of a regular expression. A companion implementation produces: multivariate generating functions for the statistics under study; a fast computation of their Taylor coefficients which yields exact values of the moments with typical application to random texts of size 30,000; precise asymptotic formulæthat allow predictions in texts of arbitrarily large sizes. Our implementation was tested by comparing predictions of the number of occurrences of motifs against the 7 megabytes amino acid database PRODOM. We handled more than 88% of the standard collection of PROSITE motifs with our programs. Such comparisons help detect which motifs are observed in real biological data more or less frequently than theoretically predicted