Hyporesponsiveness in Contact Hypersensitivity and Irritant Contact Dermatitis in CD4 Gene Targeted Mouse
- Kondo, Seiji
- Beissert, Stefan
- Wang, Binghe
- Fujisawa, Hiroshi
- Kooshesh, Fatemeh
- Stratigos, Alexander
- Granstein, Richard D.
- Mak, Tak W.
- Sauder, Daniel N.
DOIAbstract
To determine the role of CD4 molecules in the generation and regulation of contact hypersensitivity (CHS), we treated mice lacking the CD4 gene as a result of targeted disruption with dinitrofluorobenzene to induce CHS. The mutant mice lacking CD4 (CD4(−) mice) showed marked hyporesponsiveness in CHS compared with normal syngeneic C57BL/6 mice (38.3±9.0% of normal at 24h after the challenge assessed by net ear swelling; p<0.025). CD4(−) mice had a larger CD4–8– double negative T-cell receptor αβ+ cell population in the lymph nodes than did normal mice, and the increase of this cell population was observed in CD4(−) mice after sensitization. Draining lymph node cells from sensitized normal mice restored the responsiveness in CD4(−) mice, but...
Add to ORKG