Bufadienolides in in vitro derived Drimia robusta plants

The bufadienolide content of plant material derived from tissue culture of Drimia robusta Bak was determined by spectrophotometry Bufadienolides were present in callus, and in three-, six- and ten-month-old ex vitro plants. Qualitative TLC analysis showed that the tissue culture derived plants contained the same bufadienolides as did the mother plant