Development of Botryococcus Seed Culture System for Future Mass Culture
- Shimamura, Ryoji
- Watanabe, Sho
- Sakakura, Yoshio
- Shiho, Makoto
- Kaya, Kunimitsu
- Watanabe, Makoto M.
DOIAbstract
AbstractThe BOT-22 strain of Botryococcus braunii was cultured under laboratory and outdoor conditions. Medium K was developed, which decreased the cost of medium preparation to 1/30th. A polycarbonate bottle (10 L) was replaced with a polyethylene bag for cost effectiveness. Addition of nitrogen substantially increased biomass of the strain; however, the hydrocarbon content remained unchanged. Growth rates of >10 L scale cultures were much lower than those of <200mL scale flask cultures. BOT-22 grew well in outdoor cultures despite extensive fluctuations in temperature and light conditions during the day from autumn to early winter and at spring. Maximum growth rates obtained using a tube bioreactor were two times higher than those obtaine...
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