Breast histomorphometry of rats treated with estrogen and/or progestogen

SummaryObjectiveTo evaluate the breast histomorphometric changes in rats treated with estrogen and/or progestogen for a short period of time.MethodsForty oophorectomized rats were divided into four groups: GC, vehicle; GE, treated with estradiol benzoate (37.6mg/animal); GP, treated with medroxyprogesterone acetate (11.2mg/animal); and GEP, treated with estradiol benzoate (37.6mg/animal) plus medroxyprogesterone acetate (11.28mg/animal). In GE group, estradiol was administered subcutaneously for seven days; in GEP group, estradiol was administered once in a day for the first seven days and the progestogen over the next 23 days both subcutaneously. Twenty-four hours after the last hormone administration, the animals were killed upon deep anesthesia and the first inguinal breasts were removed, fixed in 10% formaldehyde and processed to be included in paraffin, with the sections being stained by hematoxylin-eosin. Morphology and the area occupied by mammary parenchyma were assessed, with the data undergoing analysis of variance followed by the Kruskal-Wallis test (p<0.05).ResultsThe control group breasts were found atrophic and, in GE and GEP group animals, typical alveoli with secretion inside are present; in progestogen-treated animals (GP), alveoli formed by large cells occupying almost the entire alveolar lumen are noted. Morpho-metric analysis showed a larger mammary parenchyma area in hormone-treated animals (GE = GP > GEP > GC; p