High-throughput PCR assays to monitor Wolbachia infection in the Dengue mosquito (Aedes aegypti) and Drosophila simulans

Lee, Siu F
White, Vanessa L
Weeks, Andrew R
Hoffmann, Ary A
Endersby, Nancy M

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Publication date

January 2012

DOI

10.1128/AEM.00069-12

Publisher

American Society for Microbiology

ISSN

0099-2240

Journal

Applied and Environmental Microbiology

Abstract

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.4 page(s

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High-throughput PCR assays to monitor Wolbachia infection in the Dengue mosquito (Aedes aegypti) and Drosophila simulans

Abstract

Extracted data

High-throughput PCR assays to monitor Wolbachia infection in the Dengue mosquito (Aedes aegypti) and Drosophila simulans

Abstract

Extracted data

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