Potential impact of dairy yeasts on the typical flavour of traditional ewes' and goats' cheeses

The contribution of Debaryomyces hansenii, Kluyveromyces lactis and Kluyveromyces marxianus strains to the typical flavour of traditional ewes' and goats' cheeses was assessed. Fourteen yeast strains were grown in liquid medium mimicking cheese composition and volatile compounds were identified by gas chromatography-mass spectrometry. Yeasts were able to produce key volatile compounds characteristic of the cheeses from which they were isolated. Inter-species and inter-strain variations were observed. Under the conditions tested, D. hansenii produced the lowest levels of volatile compounds, with large intra-strain variations. Kluyveromyces strains primarily produced esters and alcohols. K. marxianus strains were associated with the production of acids, ethyl decanoate, 1-propanol and benzaldehyde, whereas K. lactis was correlated with the presence of ketones, ethyl acetate and secondary alcohols. In conclusion, this study shows the heterogeneous potential of dairy yeasts to contribute to final cheese flavour.The authors thank grants Consolider Ingenio Fun-C-Food, CSD2007-00063, INIARM2007-00010 and AGL2012-38884-C02-01 for financial support. BP thanks CSIC for a JAE Predoc contract. The authors are grateful to the Cheese Company “Los Corrales” for kindly supplying the cheese samples.Peer reviewe