Dietary effect on the proteome of Octopus vulgaris paralarvae

Comunicación presentada en las CephsInAction MC & WGs meetings, celebradas en Lisboa, Portugal, del 25 al 27 de noviembre de 2015Nowadays, the common octopus (Octopus vulgaris) culture is hampered by massive mortalities occurring during early life-cycle stages (paralarvae). Although the causes of the high paralarvae mortality are not yet well defined and understood, the nutritional stress caused by suboptimal diets is pointed out as one of the main factors. In this study the effect of diet on paralarvae is analysed through a proteomic approach, to search for new biomarkers of nutritional stress. A total of 50 proteins showing differential expression in each condition analysed have been identified, highlighting proteins related with carbohydrate metabolism: glyceraldehyde-3-phosphate-dedydrogenase (GAPDH), triosephosphate isomerase, and NADP+-specific isocitrate dehydrogenase; with pyrimidine and beta-alanine metabolisms, as well as vitamin B5 and CoA biosynthesis: dihydropyrimidinase; energetic metabolism: arginine kinase; detoxification: glutathione-S-transferase (GST); stress: heat shock proteins (HSP70); structural constituent of eye lens: S-crystallin; and cytoskeleton: actin, actin-beta/gamma1, and beta actin. The results obtained allow defining characteristic proteomes of paralarvae depending on the diet, as well as the use of these proteins as new biomarkers to evaluate their nutritional stress.This research was supported by Ministerio de Ciencia e Innovación through the OCTOPHYS Project (AGL-2010-22120-C03). The study was also carried out under the scope of a PROMETEO I and II Projects (2010/006, 2015/085) from Generalitat Valenciana.Peer Reviewe