Association between lipid peroxidation marker, C-reactive protein, serum trace elements and fecal microbiota in lupus

Póster presentado en el VI Workshop Probióticos, Prebióticos y Salud: Evidencia Científica, celebrado en Oviedo, España, el 5 y 6 de febrero de 2015Our group has recently reported an intestinal dysbiosis, with a lower Firmicutes/Bacteroidetes ratio, in systemic lupus erythematosus (SLE), autoimmune disease in which inflammation and oxidative stress could be involved. The aim of this study was to compare the serum levels of antioxidants, malondialdehyde (MDA) and C-reactive protein (CRP), and to evaluate their association with intestinal microbiota, in 26 women suffering from SLE and 27 age-matched controls. Serum concentration of CRP was determined by enzymatic methods, folic acid and vitamin B12 by chemiluminescence; copper, zinc and selenium by inductively coupled plasma mass spectrometry (ICP-MS) and MDA and total antioxidant capacity (TAC) by spectrophotometric methods. Intestinal microbiota profile was evaluated by Ion Torrent 16S rRNA gene-based analysis. Results did not reveal increased levels of lipoperoxidation and CRP in SLE subjects compared to controls. Higher concentration of serum copper and lower of zinc was found in these patients in comparison with controls. Also, CRP was found directly associated with serum copper and Verrucomicrobia levels, whereas zinc was indirectly associated with Fimicutes. These results could be useful in the future to go deeper into the understanding of this complex disease.Peer Reviewe