14 pagesThe phosphatidylinositol (Pl)-specific phospholipase C (PLC) of Bacillus cereuswas cloned into Escherichia coli by using monoclonal antibody probes raised against the purified protein. The enzyme is specific for hydrolysis of the membrane lipid PI and Pl-glycan-containing membrane anchors, which are important structural componentsof one class of membrane proteins. The protein expressedin E. colicomigrated with B. cereus PI-PLC in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as detected by immunoblotting, and conferred PI-PLC activity on the host. This enzyme activity was inhibited by PI-PLC-specific monoclonal antibodies. The nucleotide sequence of the PI-PLC gene suggests that this secreted bacterial protein is ...
PLCBc is a 28.5 kDa monomeric enzyme that catalyzes the hydrolysis of the phosphodiester bond of pho...
Substrate specificities of phospholipases C [EC 3.1.4.3] from Clostridium novyi, Clostridium per-fri...
Phosphatidylinositol-specific phospholipase C (PI-PLC) activity was investigated in 25 different lac...
A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the...
92-99Phospholipase C (PLC) was purified to homogeneity from the culture filtrate of Bacillus cereus...
ABSTRACTObjectiveTo study characteristics of phospholipases C (PLCs), their importance for producing...
119 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.The phosphoinositide-specific...
Phospholipase A(1) activities have been detected in most cells where they have been sought and yet t...
Phospholipase A(1) activities have been detected in most cells where they have been sought and yet t...
<div><p>Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct ...
Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types ha...
Eukaryotic phosphoinositide-specific phospholipases C (PI-PLC) specifically hydrolyze phosphatidylin...
Phospholipase C activity present in the growth medium of Bacillus ceyeus was purified 2o-fold by chr...
Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar ...
A novel membrane-bound glycan-phosphatidylinositol-specific phospholipase C, which catalyzes the con...
PLCBc is a 28.5 kDa monomeric enzyme that catalyzes the hydrolysis of the phosphodiester bond of pho...
Substrate specificities of phospholipases C [EC 3.1.4.3] from Clostridium novyi, Clostridium per-fri...
Phosphatidylinositol-specific phospholipase C (PI-PLC) activity was investigated in 25 different lac...
A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the...
92-99Phospholipase C (PLC) was purified to homogeneity from the culture filtrate of Bacillus cereus...
ABSTRACTObjectiveTo study characteristics of phospholipases C (PLCs), their importance for producing...
119 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.The phosphoinositide-specific...
Phospholipase A(1) activities have been detected in most cells where they have been sought and yet t...
Phospholipase A(1) activities have been detected in most cells where they have been sought and yet t...
<div><p>Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct ...
Proteolytic enzymes have evolved several mechanisms to cleave peptide bonds. These distinct types ha...
Eukaryotic phosphoinositide-specific phospholipases C (PI-PLC) specifically hydrolyze phosphatidylin...
Phospholipase C activity present in the growth medium of Bacillus ceyeus was purified 2o-fold by chr...
Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar ...
A novel membrane-bound glycan-phosphatidylinositol-specific phospholipase C, which catalyzes the con...
PLCBc is a 28.5 kDa monomeric enzyme that catalyzes the hydrolysis of the phosphodiester bond of pho...
Substrate specificities of phospholipases C [EC 3.1.4.3] from Clostridium novyi, Clostridium per-fri...
Phosphatidylinositol-specific phospholipase C (PI-PLC) activity was investigated in 25 different lac...