Somatic embryogenesis and plant regeneration in immature zygotic embryo, ovule, and anther filament cultures of Chinese cabbage

Immature zygotic embryos, ovules, and anther filaments of Chinese cabbage were cultured on Murashige and Skoog's (MS) medium supplemented with mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D). Up to 80% of the zygotic embryos, 17% of the ovules, and 13% of the filaments produced somatic embryos with or without intervening calli. Upon transfer to MS basal medium, most of the somatic embryos developed into plantlets. Plantlets were transplanted to potting soil and grown in a phytotron.ope