The use of alternative sources for the production of bio-ethanol and the application of natural preservation for the prevention of spoiling distillers grains

Ethanol was made from potatoe and their by-products by using different enzymes for mass saccharification process. Safety of produced ethanol was evaluated by examination of higher alcohols and other fusel compounds. Lactic acid bacteria were used for distillers grains (DGGS) conservation. Biogenic amines and microbiological parameters of (DGGS) were evaluated. According to our results, different enzymes have different influence on technological and ethanol parameters and yield. The highest yield of ethanol was obtained from samples prepared with Stargen, Depol 692 ir Ecopulp Tx 200 - A, as well as in control samples, without enzymes. The highest concentration of fusel compounds was found in a samples L3 (81920.7 mg/l), which were produced by using Stargen. The lowest concentration of fusel compounds was found in samples B4 (4835.2 mg/l), which were produced by using Ecopulp Tx-200 A. Biogenic amines formation in DGGS were affected by enzymes used for saccharification of the raw material. The highest total biogenic amines content was found in control samples B5 (105.5 mg/kg). The lowest biogenic amines content was found in samples B2 (7.8 mg/kg), which were produced by using Depol 680 P. DGGS fermentation with selected lactic acid bacteria could be alternative for preservations, to increase DGGS nutritional value and their positive impact on the health of animals, because it would contain the recommended quantity of lactic acid bacteria (more than 106)