Pig‘s calpastatine gene polymorphism linkage to a different muscle fenotip

Research methodology. The objective of this research was to investigate the influence of pigs' calpastatine gene to the muscle growth. Research methodology: This research was performed at LUHS Biological Systems and Genetic Research Institute at K. Janušauskas Animals Genetics Laboratory. Fattening and meat traits data was obtained from Kaunas Department of Public Pigs Control Fattening Station. For this research 47 domestic pigs were selected. They belong to Duroc, Landrace, Yorkshire, Pietrain, Lithuanian whites, and Large white pigs breeds. DNA used for this research was extracted from plucked hair. PGR products were cut using restriction cropping ferments HinfI, MspI and RsaI. Results and conclutions. Results and conclusions. Calpastatine gene polymorphism research performed on different pig breeds has shown, that different genotype frequency is found in different breeds. Six alleles (A, B, C, D, E, F) and six different genotypes (AB, BB, CD, DD, EE, EF) were identificated in three polymorphic places. Using the received results, we have concluded, that CAST/MspI CD genotype pigs had larger back muscle area and ham mass, compared to other calpastatine genotype pigs, CAST/HinfI BB genotype pigs were the leanest. Estimating CAST gene's influence to fattening traits, we have come to conclusion, that during the fattening period, largest amount of forage and biggest overweight was achieved by pigs with CAST/HinfI BB genotype. In the breed of Large Whites CAST/HinfI locus, 50 percent of pigs had BB genotype, AA genotype was not found. DD genotype dominated in CAST/MspI locus (81,2 percent), AA genotype was not found. 87,5 percent of pigs had EE genotype