Superoksido anijonų išsiskyrimas iš periferinio kraujo leukocitų sergant parodontitu ir pirmojo tipo cukriniu diabetu

The involvement of reactive oxygen species in periodontal diseases is unclear. The aim of present study was to explore oxidative function of neutrophil leukocytes of patients with severe periodontitis who have type 1 diabetes mellitus, and control subjects with healthy periodontal tissues and without systemic diseases. Materials and methods. The leukocytes for present investigation were obtained from peripheral venous blood of 38 patients with severe periodontitis who have type 1 diabetes mellitus and 27 control subjects. The maximal lucigenin-dependent chemiluminescence and peak time values of neutrophils stimulated with non-opsonized Escherichia coli ATCC 25922 and Staphylococcus aureus 256 were measured. Results. The maximal lucigenin-dependent chemiluminescence of neutrophils of patients with severe periodontitis stimulated with non-opsonized E. coli and S. aureus was much higher than that in control subjects (p<0.001). In both affected and healthy patients, chemiluminescence of neutrophil leukocytes reached its peak value at similar time. The maximum value of chemiluminescence of leukocytes stimulated with non-opsonized E. coli in both studied groups was reached statistically significantly earlier than when stimulating with non-opsonized S. aureus bacteria (p