The investigation of 1,4 - naphtoquinone derivatives effect to glioblastoma C6 cuture cells

The aim of research – to investigate and compare the effect of different concentrations of three different 1,4-naphthquinones: plumbagin, menadione and lawsone effectiveness on rat glioblastoma cell culture viability, and assess their impact to the GMB cell proliferation. Methods: Cell viability was evaluated using propidium iodide and Hoechst 33258 assay, additionally cell viability was assessed by measuring the ability of cells to metabolize MTT dye. Cell staining with propidium iodide and Hoechst 33258 also was used to investigate anti-proliferative effect. Goals of the study: 1. To identify and compare the effect of different concentrations of 1,4-naphthoquinone derivatives on cell viability. 2. To investigate and compare the effect of different concentrations of 1,4-naphthoquinone derivatives on cells proliferation. Results: Our results have shown that investigated 1,4-naphtoquinones induced dose-depend reduction in viability of C6 cells. The IC50 values after 24 h were approximately 7.7 ± 0.28μM of plumbagin, 8.6 ± 0.75 μM of menadione and 1368 ± 280 μM of lawsone. Furthermore, propidium iodide/Hoechst assay confirmed that increased concentrations of 1,4-naphthoquinones have effectively lowered viable cell and increased non-viable cell ratio. Incubation of cells with 1-5 µM of menadione and 1-4 µM of plumbagin reduced cell proliferation by 16-27 %, lawsone reduced cell proliferation by 48% after incubation of cells with 500–1000 µM. Conclusions: 1,4 - naphthoquinones (plumbagin, menadione, lawsone) induced a dose-dependent reduction in viability and proliferation of C6 cells