Comparison of sample preparation methods in toxicological analysis

In this master’s thesis are examined solid phase extraction and liquid - liquid extraction methods. Codeine has been extracted by using these methotds, efficiencies of extraction methods were determined by UV spectrophotometry. The aim of this this master’s thesis is to determine the optimal sample preparation methodologies for toxicological analysis, codeine proper isolation of biological media - plasma, urine, and to compare the effectiveness of these methods. After assessment of the data, it can be said that the extraction of codeine from urine, efficiencies were similar extracting by using liquid - liquid extraction and solid phase extraction. Codeine is more efficiently extracted from blood plasma by solid phase extraction method than liquid - liquid extraction method