Fusarium spp. influence on mycotoxins accumulation in the grain during storage

The Object of the work: testing samples taken from granaries located in the X and XX Vilkaviškis district, taken 30 wheat and 30 barley samples. The aim of the work: changes in the grain and dynamics of fusariotoxins while granaring and to evaluate effectiveness of preventive measures against spreading of Fusarium spp. in grain. Reasearch objects:to assess fungi contamination in grain (wheat and barley) while granaring; to evaluate prevalence of fusariotoxins (deoxynivalenol, zearalenone, T–2 toxin ) in the grain while granaring;to evaluate effectiveness of preventive measures in X farms against spreading of Fusarium spp. in the grain. The results and their discussion: carried out quantitative wheat and barley contamination with fungi rudiment, found that ultimate „Ūla“ species barley contamination determined in 2013 March 113±10,4*103 KSV/g. Ultimate „Luokė“ species barley contamination with fungi rudiment determined 2012 March 109±19,9*103 KSV/g. Ultimate contamination of wheat species „Triso“ as well was determined 2012 March 204±7,8*103 KSV/g, mean while ultimate contamination of species „Munk“ was determined 2012 March 128±10,8*103KSV/g. Compared spreading in barley of Fusarium spp. in granaries X and XX species „Ūla“ and „Luokė“ prevalence found: ultimate contamination with fungi rudiment in barley „Ūla“ range 80±0,0 %, species „Luokė“ 80±0,0 %. Ultimate external contamination of fusariosis in species „Ūla“ range 73±0,44 % (2012 January) and 73±3,33 % (2012 September), species „Luokė“ 70±5,77 % (2012 August) and 70±11,55 % (2012 September). Compared wheat species „Triso“ and „Munk“ samples taken from farms X and XX research results found that contamination with Fusarium spp. was higher in wheat species „Munk“. Ultimate internal contamination range 77±8,82 %, external 93±26,67%, mean while species „Triso“ ultimate internal contamination 60±5,77 % , external 87±21,86 %. The highest DON concentration in wheat was found in september 2011, range 1200 μg/kg. This concentration determined in wheat species „Triso“ granary XX. Highest T–2 toxin concentration determined barley species „Luokė“ granary X – 200 μg/kg. The highest ZON concentration in wheat was found in wheat species „Triso“ XX granary – 870 μg/kg. The conclusion: As the results show, prevalence of Fusarium spp. in granaries grain has great impact to the emergence and accumulation of mycotoxins, spread of Fusarium spp. in warehouse grain impacted by grain dampness, impurity, near stored crops and general condition of granaries