Semisolid systems with bees products manufacturing and biopharmaceutical evaluation

The aim of work: Formulate and analyze semisolid preparations with bees products and evaluate their quality by biopharmaceutical researches. Main tasks: 1. Select the proper form of propolis as an active material in formulated semisolid systems; 2. Formulate stable semisolid systems with bees products (beeswax, propolis, honey) and evaluate their quality by investigation of these parameters: homogenity, pH value, antioxidant activity; 3. Perform biopharmaceutical evaluation of semisolid systems with bees products and identify the factors that influence release the total amount of phenolic compounds; 4. Evaluate quality of semisolid systems with bees products based on active compounds (phenolic acids, vanillin) in vitro release test results. Semisolid systems with bees products are analyzed by using spectrophotometric and capillary HPLC methods for analysis of total phenolic compounds measuring, DPPH• free radical scavenging activity measuring according to trolox. Quality of the systems was evaluated by measuring pH values and by testing release of active compounds in vitro. The active substance propolis is inserted into semisolid systems by thick or liquid (in gels) extract form. Stable system of the Group I semisolid systems is formulated when beeswax and sunflower oil ratio is less than 1:5. Beeswax and honey in systems affect the release of active compounds - increasing the wax content, the active compounds release of the systems is reduced, adequate quantity of honey increases the release of active compounds from semisolid systems. The largest amount of the active compounds is released from semisolid systems with honey and propolis after 6 h from the beginning of the study (13.46 ± 0.26 per cent.). Semisolid systems pH value is from 4,07±0,034 till 5,383±0,162. Semisolid systems with the largest amount of the active compound (propolis) has the best antioxidant activity. Honey compound in semisolid systems strengthens the antioxidant activity. HPLC method showed that in semisolid systems dominate p-coumaric, ferulic acid and vanillin. In semisolid systems with honey are found higher levels of these active compounds, so studies demonstrated that honey can be used as an active compounds in semisolid systems