Influence of long storage and qualitative sperm parameters on motily, velocity and viability of boars spermatozoa Influence of long storage and qualitative sperm parameters on motily, velocity and viability of boars spermatozoa

The present study was conducted to determine the influence of long storage and qualitative sperm parameters on motily, velocity and viability of boars spermatozoa and also a correlation of motility, velocity, viability and morphology. 33 ejaculates from a different AI boars extended in Androhep plus were used. Sperm concentration and morphology were registered. Motility, viability, velocity and acrosome integrity were assessed after storage at 17°C for 6, 30, 54, 78, 102 and 150 h. Sperm velocity was assesed by SCA, viability by hypo-osmotic swelling test by using 100 mOsm/L sodium citrate fructose solution. Motility, morphology and acrosome integrity were assesed using conventional semen evaluation methods. Storage time had a significant influence on motility, viability and velocity while they both declined and had a significant correlation (p≤0.01) with each other. To determine the influence of abnormal sperm morphology on motility, viability and velocity each semen sample was divided into two groups. First group was with less than 10 percent of abnormal morphology, while the second group was with the greater than 10 percent of abnormal morphology. The mean of the 102 h sperm positive to HOT test on the first group was greater by 8,7 ± 1,2 % than the second group and after a 102 h the second group was below the critical 40 % of sperm positive limit, while the first group had still 45,93 % sperm positive to HOT test. The motility on the second group reached the critical 60 % motile limit at 78 h, while the first group reached it 24 h later at 102 h. Statistical analysis of the data revealed a significant (p≤0.01) positive correlation between motility and sperm viability determined by percentage of HOT test positive spermatozoa. There was a significant (p≤0.05) negative correlation betwen the abnormal head morphology with HOT test and cytoplasmic droplets with motility. The velocity (VCL, VAP, VSL) declined while LIN and STR inclined and WOB did not showed any significant changes throughout the experiment. There was no significant influence of sperm morphology on velocity. Surprisingly, the influence of spermatozoa concentration was just on the VSL, LIN and STR with a significant (p≤0.05) positive correlations. Means for percentage of spematozoa with normal acrosomes showed a small decline from 99.73 % after 6 h of storage to 98.91 % at 150 h. However, there was a significant (p≤0.05) negative correlation with abnormal head morphology