Evaluation of benzimidazole and dihidropyridine effects on vascular segments and papillary muscle isometric function

This study aim was to evaluate the effects of 1,4-dihydropiridines and benzimidazole deriva¬tives on the isometric function of guinea pig papillary cardiac muscles and the contraction and relaxation of vascular segments in humans. The experiments were carried out on isolated samples of human great saphenous vein (v. saphena magna) and internal thoracic artery (a. tho¬racica interna). The vein and arteries samples were taken from patients who underwent coronary artery bypass. The study was approved by the Regional Ethics Committee of the Biomedical Research on 05/11/2010, license No. BE-2-64, Kaunas, Lithuania. The inotropic activity and transmembrane AP duration of the dihydropyridine derivatives were evaluated on the guinea-pig papillary muscles and aorta vascular samples. Synthesis of 1,4-dihydropyridine derivatives were performed in Latvian Organic Synthesis institute. During this study were performed the evaluation of 1,4-dihydropyridines and benzimidazole derivatives on contraction force and action potential in guinea pig papillary muscles. Effects of 1,4-dihydropyridines and benzimidazole derivatives on contraction and relaxation of the segments of the great sa¬phenous vein (v. saphena magna) and internal thoracic artery (a. tho¬racica interna) in humans, the extra-cellular concentration of Ca2+ and the effect of the studied compounds and were evaluated. Also assessment of preventive effect of the calcium channel blockers and benzimidazole derivative on contraction of isolated vein samples was performed