Evaluation of testicular sperm CRISP2 as a potential target for contraception
- Weigel Muñoz, Mariana
- Ernesto, Juan Ignacio
- Bluguermann, Carolina
- Cohen, Debora Juana
- Busso, Dolores
- Battistone, Maria Agustina
- Cuasnicu, Patricia Sara
DOIAbstract
Cysteine-rich secretory protein 2 (CRISP2) is a testicular sperm protein proposed to be involved in fertilization. With the aim of examining the relevance of CRISP2 for fertility and its potential use as a target for contraception, in the present work, male and female rats were immunized with recombinant CRISP2 coupled to maltose-binding protein (MBP) and evaluated for their subsequent fertility. As controls, animals were injected with either MBP or recombinant CRISP1. Enzyme-linked immunosorbent assay of sera collected at different intervals after immunization indicated that CRISP2 immunization raised specific antibodies in both sexes, with levels that increased as a function of time. Western blot studies revealed that anti-CRISP2 sera wer...
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