Add to ORKGin primer extension (PEX) reaction, a DNA polymerase is used to extend specifically a primer that anneals immediately adjacent to the variant nucleotide. The reaction allows highly specific detection of point mutations and single nucleotide polymorphisms (SNPs). Because all SNPs can be analyzed with high specificity at the same reaction conditions, PEX is a promising reaction principle for multiplex high-throughput genotyping assays. This review discusses the possible applications of PEX and different approaches of primer extension reactions
<p><b>A.</b> Schematic representation of the strategy of primer extension used for detecting pause s...
DNA polymerases are used in a plethora of biotechnical applications, especially in the polymerase ch...
In spite of the numerous advances in the area of sequencing, the actual procedure is still expensive...
in primer extension (PEX) reaction, a DNA polymerase is used to extend specifically a primer that an...
Arrayed primer extension reaction (APEX) is a straightforward and robust enzymatic genotyping method...
<p> Polymerase extensions of 27t/11p<sup>*</sup> or 11p were performed as described in MATERIALS and...
Multiplex PCR method is a powerful technology as a potent tool to amplify specific DNA fragments fro...
Single nucleotide polymorphisms (SNP) are the most frequent form of DNA sequence variation in the hu...
<p>(A) Principle of the single-nucleotide primer extension method illustrated through analysis of a ...
PCR is used extensively in many areas of DNA testing. Primer dimers (PD) form when two primers hybri...
Single-nucleotide polymorphism (SNP) analysis is a powerful tool for mapping and diagnosing disease-...
Studies on genetic variation can reveal effects on traits and disease, both in humans and in model o...
We have investigated the possibility of genotyping single nucleotide polymorphisms (SNPs) by primer ...
Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including ...
The polymerase chain reaction (PCR) is a method for the selective amplification of DNA or RNA segmen...
<p><b>A.</b> Schematic representation of the strategy of primer extension used for detecting pause s...
DNA polymerases are used in a plethora of biotechnical applications, especially in the polymerase ch...
In spite of the numerous advances in the area of sequencing, the actual procedure is still expensive...
in primer extension (PEX) reaction, a DNA polymerase is used to extend specifically a primer that an...
Arrayed primer extension reaction (APEX) is a straightforward and robust enzymatic genotyping method...
<p> Polymerase extensions of 27t/11p<sup>*</sup> or 11p were performed as described in MATERIALS and...
Multiplex PCR method is a powerful technology as a potent tool to amplify specific DNA fragments fro...
Single nucleotide polymorphisms (SNP) are the most frequent form of DNA sequence variation in the hu...
<p>(A) Principle of the single-nucleotide primer extension method illustrated through analysis of a ...
PCR is used extensively in many areas of DNA testing. Primer dimers (PD) form when two primers hybri...
Single-nucleotide polymorphism (SNP) analysis is a powerful tool for mapping and diagnosing disease-...
Studies on genetic variation can reveal effects on traits and disease, both in humans and in model o...
We have investigated the possibility of genotyping single nucleotide polymorphisms (SNPs) by primer ...
Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including ...
The polymerase chain reaction (PCR) is a method for the selective amplification of DNA or RNA segmen...
<p><b>A.</b> Schematic representation of the strategy of primer extension used for detecting pause s...
DNA polymerases are used in a plethora of biotechnical applications, especially in the polymerase ch...
In spite of the numerous advances in the area of sequencing, the actual procedure is still expensive...