One of the biggest challenges of modern cosmology is to gain a more precise knowledge of the dark energy and the dark matter nature. Fortunately, the dark matter can be traced directly through its gravitational effect on galaxies shapes. The European Spatial Agency Euclid mission will precisely provide data for such a purpose. A critical step is analyzing these data will be to accurately model the instrument Point Spread Function (PSF), which the focus of this thesis.We developed non parametric methods to reliably estimate the PSFs across an instrument field-of-view, based on unresolved stars images and accounting for noise, undersampling and PSFs spatial variability. At the core of these contributions, modern mathematical tools and concept...