B-Cell Non-Hodgkin's Lymphoma Cell Line (Karpas 1106) With Complex Translocation Involving 18q21.3 but Lacking BCL2 Rearrangement and Expression

A B-cell non-Hodgkin's lymphoma (B-NHL) cell line (Karpas 1106) with an unusual three-way translocation involving 18q21.3 has been derived from a patient with mediastinal lymphoblastic B-NHL. Although conventional cytogenetics showed a derivative 18q- identical to that seen in cases with t(l4;18)(q32.3;q21.3), no translocations of either chromo-some 14 could be detected. Instead fluorescent in situ hy-bridization analysis using a chromosome-l8 paint showed that the segment 18q21.3-18qter had become sandwiched on a derivative chromosome X between segments Xqter-c-Xq28 and 13q12-qter, with the centromeric site of 18q21.3 subband juxtaposed to the X sequences. Pulsed-field DNA HROMOSOMAL translocation t ( 14; 1 Q(q32.3; q2 1.3) was first identified in follicular B-cell non-Hodgkin's lymphoma (B-NHL) in 1976 by Fukuhara and colleagues.&apos